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http://dx.doi.org/10.25673/120305| Title: | Mechanisms of the MRTF-A controlled myofibroblastic differentiation and its influence on the tumour propagating function of mesenchymal stromal cells |
| Author(s): | Werner, Sara |
| Referee(s): | Posern, Guido Müller, Lutz P. Wagner, Wolfgang |
| Granting Institution: | Martin-Luther-Universität Halle-Wittenberg |
| Issue Date: | 2024 |
| Extent: | 1 Online-Ressource (VI, 109 Seiten) |
| Type: | Hochschulschrift |
| Type: | PhDThesis |
| Exam Date: | 2025-05-12 |
| Language: | English |
| URN: | urn:nbn:de:gbv:3:4-1981185920-1222631 |
| Abstract: | MRTF-A is a key transcriptional regulator of myofibroblastic differentiation. This thesis investigates its role in primary human mesenchymal stromal cells (MSCs) in colorectal cancer. TGF-β1, secreted by colorectal cancer cells such as HCT-8, activates MRTF-A, driving the expression of contractile and extracellular matrix genes and establishing a distinct myofibroblastic signature. These changes resemble features of cancer-associated fibroblasts (CAFs). Functionally, MRTF-A enhances the tumour-supportive phenotype of MSCs, as shown in vitro and in a colorectal cancer xenograft model. MRTF-A depletion via siRNA and shRNA reduces both myofibroblastic traits and pro-tumourigenic effects, underscoring MRTF-A as a central mediator of TGF-β–driven stromal activation. |
| URI: | https://opendata.uni-halle.de//handle/1981185920/122263 |
| Open Access: |  Open access publication |
| License: |  (CC BY 4.0) Creative Commons Attribution 4.0 |
| Appears in Collections: | Interne-Einreichungen |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Dissertation_MLU_2025_WernerSara.pdf | 11.31 MB | Adobe PDF | View/Open |