Assessment of the Effectiveness of Prolidase and Study of the Impact of Certain Antioxidants in Individuals Suffering from Beta Thalassemia in Anbar Province

Abstract

Thalassemia is a common hereditary disease that is transmitted from parents to their children. As a result, many individuals do not follow the recommended guidelines, particularly before marriage. Early screening is considered an important measure to raise awareness among individuals for the early detection of thalassemia. The current study included 60 individuals (males and females) diagnosed with thalassemia, aged between 5 and 20 years, identified by specialists in hematology at Al Ramadi Hospital in Al Anbar Governorate, and 30 individuals (males and females) aged 5 to 20 years who are not affected by thalassemia, serving as a control group. Samples were collected at Al Ramadi Hospital for Women and Children / Thalassemia Unit.We discussed the effect of the disease on the activity of the Prolidase, which was found to significantly increase when exposed to this pathological condition (thalassemia). Therefore, we can rely on the estimation of elevated prolidase activity as a biomarker for the onset of health deterioration in individuals affected by thalassemia, thus preventing progression to advanced stages of the disease. The study aimed to estimate the effectiveness of the Prolidase in patients with thalassemia and compare it with a control group to understand the reason for the elevated enzyme activity in the patients. The measurement of levels of certain antioxidants, including (CAT, D3, MAD, GSH). The results showed a significant decrease in levels of (D3, GSH) at a significance level of (P ≤ 0.05) in patients compared to the control group. At the same time, there was a significant increase in levels of (MAD, CAT) at a significance level of (P ≤ 0.05) in the serum of patients compared to healthy individuals.